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Structure/Function Analysis of the Hif1 Histone Chaperone in Saccharomyces cerevisiae

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posted on 2021-05-22, 14:00 authored by Nora Saud Dannah
Understanding the regulation of chromatin structure is a vital aspect of molecular biology regarding its influences on biological processes such as DNA replication, transcription (gene expression), DNA repair, chromosome segregation and recombination. In the budding yeast Saccharomyces cerevisiae, a histone chaperone called Hif1 has been found in the nuclei as having a functional role in chromatin assembly. Hif1 is a homolog of the human protein NASP that is involved in the maintenance of genome stability. Previously, Hif1 has been shown to physically interact with Hat1, Hat2 and H3/H4 to form the NuB4 complex directly involved in chromatin assembly. A molecular genetic approach was conducted to determine which domain of Hif1 is involved in the interaction with the HAT1 complex.

History

Language

English

Degree

  • Master of Science

Program

  • Molecular Science

Granting Institution

Ryerson University

LAC Thesis Type

  • Thesis

Year

2014

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    Molecular Science (Theses)

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