Examining Plasmid Transfer of pJP4 in a Mixed Community Biofilm Formed in a Microcosm Simulating a Porous Aquifer

In this work, a lab-scale microcosm was designed and used to simulate a porous groundwater aquifer. A minimal media acted as the non-selective pressure for plasmid transfer, while a minimal media with gentamicin acted as the selective pressure. PCR, plate counts and confocal laser scanning microscopy were used to monitor transconjugant and donor persistence in the microcosm. The donor was identified through