Toronto Metropolitan University
Browse

Examining Plasmid Transfer of pJP4 in a Mixed Community Biofilm Formed in a Microcosm Simulating a Porous Aquifer

Download (2.44 MB)
thesis
posted on 2021-05-22, 17:18 authored by Floriana Postelnik
In this work, a lab-scale microcosm was designed and used to simulate a porous groundwater aquifer. A minimal media acted as the non-selective pressure for plasmid transfer, while a minimal media with gentamicin acted as the selective pressure. PCR, plate counts and confocal laser scanning microscopy were used to monitor transconjugant and donor persistence in the microcosm. The donor was identified through

History

Language

English

Degree

  • Master of Science

Program

  • Molecular Science

Granting Institution

Ryerson University

LAC Thesis Type

  • Thesis

Year

2012

Usage metrics

    Molecular Science (Theses)

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC