Ozone pretreatment of humid wheat straw for biofuel production

In an attempt to maximize the amount of ozone reacting with lignin inside humid wheat straw, some of the ozone-reactive lignin degradation products were washed away before a second ozonolysis delignification stage. The total contact time for the two stages was kept the same as that for a one-stage process for comparison. A significant decrease in the Acid Insoluble Lignin (AIL) content of the straw resulted: from 13.04 wt. % (after a 30-min one-stage ozonolysis) to 9.34 wt. % (after a 30-min two-stage ozonolysis, separated by a washing step). This significant improvement was accompanied by an increase in released fermentable sugars from an enzymatic hydrolysis. The yield increased from 60% theoretical sugars to 80%. A further improvement in AIL (down to 7.36 wt. %) and released sugars (up to 90% theoretical) occurred when the moisture content (MC) of the straw entering the second stage was adjusted to the optimum value of the straw entering first stage (45 wt. %, predicted from an experimental design). The authors believe this is the first time results are published for the introduction of a two-stage process separated by a washing step.